Molecular Methods

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Malinow Molecular Methods Quantum Dots Choquet AMPAR

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Hi Guys, Here are the different labeling techniques that might be applicable with recombinant expression of AMPARs. Roughly ranked from most to least likely to succeed, separated by large vs small AMPAR N-terminal additions. The references in parentheses are for background on the technique.


Large AMPAR N-terminal addition
  • Halotag (Promega): AMPAR-enzyme, QD-Halotag substrate
  • AMPAR-streptavidin, QD-biotin
Small AMPAR N-terminal addition
  • AMPAR-FLAG, QD-anti-FLAG
  • AMPAR-biotin ligase recognition peptide, QD-biotin + biotin ligase (LuTing2013PLOSONE)
  • AMPAR-peptide A, QD-peptide B, which binds peptide A (ZhangKodadek2000NatBiotech)
  • AMPAR-unnatural amino acid azide, QD-propargyl (ChaterjeeSchultz2013PNAS)


Fluorescence Imaging


Molecular Interaction or Proximity


Microscopy


See Also