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<mediaplayer image='http://www.bradleymonk.com/w/images/d/da/Kasai_GluUncaging_S7.png' width='500' height='300'>http://www.bradleymonk.com/w/images/1/18/Kasai_GluUncaging_S7.mov</mediaplayer>
Movie S7. Two-photon imaging of the confinement of PAGFP-actin fluorescence after repetitive photoactivation and uncaging of MNI-glutamate (60 pulses of 0.6 ms duration at 1 Hz) at a point (square) distal to the apex of the spine shown in Figure 5D. The 2D images were acquired every 10 s for 7 min. Photoactivation was induced at the moment when the white square turns red. Scale bar, 1 μm.