User talk:Karishma Pratt: Difference between revisions

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(Created page with "{{Box|Receptor Protein Tags|width=45%| * fluorescent proteins * superecliptic phluorin (SEP) * photoactivatable green fluorescent protein (PAGFP) }} {{Box|Protein Quantificat...")
 
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{{Box|Receptor Protein Tags|width=45%|
{{Box|Receptor Protein Tags|width=45%|
* fluorescent proteins
* fluorescent proteins
* superecliptic phluorin (SEP)
* [[#superecliptic phluorin SEP]]
* photoactivatable green fluorescent protein (PAGFP)
* photoactivatable green fluorescent protein (PAGFP)
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}}
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* Western Blot
* Western Blot
* Quantitative Autoradiography
* Quantitative Autoradiography
* [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3339873/pdf/jove-50-2568.pdf FRAP]
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* Quantum Dot Tagging
* Quantum Dot Tagging
* Halotag (Promega): [[AMPAR]]-enzyme, QD-Halotag substrate
* Halotag (Promega): [[AMPAR]]-enzyme, QD-Halotag substrate
* AMPAR-streptavidin, QD-biotin
* streptavidin, QD-biotin
* AMPAR-FLAG, QD-anti-FLAG
* FLAG, QD-anti-FLAG
* AMPAR-biotin ligase recognition peptide, QD-biotin + biotin ligase (LuTing2013PLOSONE)
* biotin ligase recognition peptide
* AMPAR-peptide A, QD-peptide B, which binds peptide A (ZhangKodadek2000NatBiotech)
* unnatural amino acid azide, QD-propargyl
* AMPAR-unnatural amino acid azide, QD-propargyl (ChaterjeeSchultz2013PNAS)
* [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3690942/ sptPALM]
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* [http://en.wikipedia.org/wiki/F%C3%B6rster_resonance_energy_transfer FRET] or BRET
* [http://en.wikipedia.org/wiki/F%C3%B6rster_resonance_energy_transfer FRET] or BRET
* [http://en.wikipedia.org/wiki/Bimolecular_fluorescence_complementation BiFC]
* [http://en.wikipedia.org/wiki/Bimolecular_fluorescence_complementation BiFC]
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{{Box|Cell Culture|width=45%|
* [http://www.lifetechnologies.com/us/en/home/references/protocols/neurobiology/neurobiology-protocols/isolation-culture-and-characterization-of-cortical-and-hippocampal-neurons.html Rat Hippocampus Neurons E18]
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{{Clear}}
{{Clear}}
==superecliptic phluorin SEP==
superecliptic phluorin (SEP) are used to do this...

Latest revision as of 22:37, 30 September 2013

Receptor Protein Tags

Protein Quantification

  • Western Blot
  • Quantitative Autoradiography
  • FRAP

Molecular Tracking

  • Quantum Dot Tagging
  • Halotag (Promega): AMPAR-enzyme, QD-Halotag substrate
  • streptavidin, QD-biotin
  • FLAG, QD-anti-FLAG
  • biotin ligase recognition peptide
  • unnatural amino acid azide, QD-propargyl
  • sptPALM

Protein Interaction

  • FRET
  • Two Hybrid Screening
  • FRET or BRET
  • BiFC

Cell Culture


superecliptic phluorin SEP

superecliptic phluorin (SEP) are used to do this...