|
|
| Line 1: |
Line 1: |
| ==Quantitative Review==
| | #REDIRECT [[Neural Anatomy]] |
| | |
| [[File:Dendrite 3D.png|thumb|left|400px|[http://synapses.clm.utexas.edu/anatomy/dendrite/tables/table1.stm Harris Website]]] | |
| | |
| {{Box|font=120%|width=45%|float=left|text=12px|The Size of Dendrites|
| |
| ;adapted from [http://www.ncbi.nlm.nih.gov/pubmed/17243894 Sheng and Hoogenraad (2007)] {{Fig|[[File:Spine.png|1000px]]}}<br>
| |
| * Dendrite: 1–10 spines per 10 μm <br>
| |
| * Spines: 0.5–2 μm in length <br>
| |
| * PSD: 100 - 300 nm diameter<br>
| |
| * PSD95: within 12 nm of surface <br>
| |
| | |
| ;adapted from [http://synapses.clm.utexas.edu/anatomy/dendrite/tables/table1.stm Harris] {{Fig|[[File:Dendrite Table.png]]}}<br>
| |
| * proximal dendrite diameter: 1 - 3 µm
| |
| * distal dendrite diameter: 0.2 - 2 µm
| |
| * dendrite length: 2000 - 9000 µm
| |
| * dendrite tip to soma: 100 - 200 µm
| |
| * dendrites at soma: 1 - 5
| |
| * dendrite branches (granual): 10 - 30
| |
| * dendrite branches (purkinje): 400-500
| |
| | |
| }}
| |
| | |
| {{Box|font=120%|width=43%|float=left|text=12px|Particle Counts|
| |
| ;adapted from [http://www.ncbi.nlm.nih.gov/pubmed/17243894 Sheng and Hoogenraad (2007)] {{Fig|[[File:Spine.png|1000px]]}}<br>
| |
| * PSD: 10,000 proteins (or 100 copies of 100 proteins)<br>
| |
| * CaMKIIα: 7.4% <br>
| |
| * CaMKIIβ: 1.3%<br>
| |
| * SynGap: 2.1 pmol/20 μg<br>
| |
| * NMDAR: 20 proteins<br>
| |
| * AMPAR: 15 proteins<br>
| |
| * GluR: 60 subunits, 15 tetramers, 80% or 12 GluR1/GluR2 heteromers<br>
| |
| * PSD95: within 12 nm of surface <br>
| |
| | |
| }}
| |
| | |
| {{Box|font=120%|width=43%|float=left|text=12px|Diffusion Rates|
| |
| ; from Choquet 2010 {{Fig|[[File:ChoquetDiffusionRate1.png]]}}<br>
| |
| * extrasynaptic: 0.1 µm<sup>2</sup>⁄s
| |
| * synaptic: 0.05 µm<sup>2</sup>⁄s
| |
| * synaptic after glu/gly: 0.01 µm<sup>2</sup>⁄s
| |
| | |
| }}
| |
| | |
| {{Box|font=120%|width=43%|float=left|text=12px|Images|
| |
| ; From [http://www.ncbi.nlm.nih.gov/pubmed/17243894 Sheng and Hoogenraad 2007]
| |
| * Spine morphology {{Fig|[[File:Spine.png|1000px]]}}
| |
| | |
| ; From [http://www.ncbi.nlm.nih.gov/pubmed/22357909 Harris KM and Weinberg 2012]
| |
| * Spine morphology {{Fig|[[File:Synaptic Buton.png]]|3D reconstruction of a proximal CA3 pyramidal cell dendrite (blue) and a large mossy fiber bouton (translucent yellow). The cut-away in C2 shows synapses (red) onto multiple dendritic spines, some of which are highly branched. The bouton also forms nonsynaptic cell adhesion junctions (fuchsia).}}
| |
| * Hippocampal dendrite {{Fig|[[ File:Hippocampal Neuron.jpg]]}}
| |
| | |
| }}
| |
| | |
| | |
| | |
| | |
| {{Box|font=120%|width=43%|float=left|text=12px|Choquet 2007 Real Time Receptor Diffusion|
| |
| [http://bradleymonk.com/media/QdotsRealTime.mov This link is to a video] of an optimized version from [http://www.cell.com/neuron/supplemental/S0896-6273%2807%2900289-9 Choquet 2007] (seen below). The dimensions in both 10 x 10 µm. The original version below is run at 4x real-time. The linked video above is slowed to 1x real-time, and all analysis is done at 1:1 video to real-time speed.
| |
| | |
| <html>
| |
| <iframe src="http://bradleymonk.com/media/QD1/vid1.html"
| |
| height="470" width="470" frameborder="0" seamless="seamless" style="float:left">
| |
| </iframe>
| |
| </html>
| |
| | |
| }}
| |
| | |
| {{Box|font=120%|width=43%|float=left|text=12px|Choquet 2007 Real Time Receptor Diffusion Analysis|
| |
| * The video represents a 10µm × 10µm section scaled to a 535px × 535px video.
| |
| ** {{Button|1<sub>'''µm'''</sub> : 53.5<sub>'''px'''</sub>}}
| |
| * The analysis below documents one instance of Qdot diffusion, between the 6s-7s time points.
| |
| * This instance was chosen because of the clarity of motion and no Qdot flicker.
| |
| * The Qdot (center) moves from pixel location (X:291, Y:302) at 6.78s to (X:319, Y346) at 6.98s
| |
| ** That is a distance of 52.2px in 200ms
| |
| ** Qdot velocity: {{Button|Q<sub><var>v</var></sub> ≈ 1<sub>'''µm'''</sub> ⁄ 200<sub>'''ms'''</sub>}}
| |
| ** Note this diffusion rate of 5µm/s is 10-fold higher than the median diffusion rate reported above.
| |
| ** An upper bound of 5µm/s means that receptors can move between synapses in fractions of a second.
| |
| | |
| <big>Figures:
| |
| :{{Fig|[[File:Choquet Diffusion Rate Analysis1.png]]}}
| |
| :{{Fig|[[File:Choquet Diffusion Rate Analysis2.png]]}}
| |
| :{{Fig|[[File:Choquet RT1.png]]}}
| |
| :{{Fig|[[File:Choquet RT2.png]]}}
| |
| </big>
| |
| | |
| }}
| |
| | |
| | |
| {{Box|font=120%|width=95%|float=left|text=12px|Receptor Diffusion Rate Best Estimates|
| |
| * GABAA: .01 - .05 µm<sup>2</sup>/s {{Fig|[[File:Choquet1 2010.png]]|[http://www.sciencedirect.com/science/article/pii/S0896627310004654 Choquet 2010]}}
| |
| }}
| |
| | |
| | |
| | |
| | |
| | |
| | |
| {{PageHead|[[Malinow]]|[[Molecular Methods]]|[[Quantum Dots]]|[[Choquet]]|[[AMPAR]]}}
| |
| [[Category:Malinow]] [[Category:ReDiClus]]
| |