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| {{PageHead|[[Malinow]]|[[Molecular Methods]]|[[Quantum Dots]]|[[Choquet]]|[[AMPAR]]}} | | {{Style|size=150%|align=center|font=Century Gothic|background=#E0DCC3|color=#07677A|pad=8px|margin=6px| |
| | | CHECK OUT MY HOMEPAGE ([http://bradleymonk.com bradleymonk.com]) FOR BIO, CV, AND CONTACT INFO |
| ==Overall Summary==
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| {{ExpandBox|Complete Picture|
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| [[File:Choquet 2004b.png|thumb|left|500px|
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| * TTX - block neural activity <br>
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| * KCl - envoke neural activity <br>
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| * TPA - stimulate PKC
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| ]]{{Clear}}
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| ;2004
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| {{Box|width=20%|KCl|
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| ;synaptic
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| * {{Up}}{{Nc}} GluR2
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| * {{Nc}} NMDAR
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| ;extrasynaptic
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| * {{Up}} GluR2
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| * {{Nc}} NMDAR
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| }}
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| {{Box|width=20%|TTX|
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| ;synaptic
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| * {{Nc}} GluR2
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| * {{Nc}} NMDAR
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| ;extrasynaptic
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| * {{Dn}} GluR2
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| * {{Nc}} NMDAR
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| }}
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| {{Box|width=20%|TPA-PKC|
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| ;synaptic
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| * {{Up}} GluR2
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| * {{Up}} NMDAR
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| ;extrasynaptic
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| * {{Dn}} GluR2
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| * {{Up}} NMDAR
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| }}
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| {{Clear}}
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| ;2003
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| {{Box|width=20%|Glutamate|
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| ;synaptic
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| * {{Up}} GluR2
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| ;extrasynaptic
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| * {{Nc}} GluR2
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| }}
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| {{Box|width=20%|Calcium|
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| ;synaptic
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| * {{Dn}} GluR2 mobility
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| * {{Up}} GluR2 expression
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| ;extrasynaptic
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| * no observations
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| }}
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| {{Clear}}
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| ;2007
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| {{Box|font=120%|width=20%|Silenced (vs active) synapses|
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| * {{Dn}} (50%) GluR1
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| * {{Nc}} PSD-95
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| * {{Nc}} presynaptic VGLUT1
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| * {{Nc}} presynaptic bassoon
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| * {{Nc}} puncta size of PSD-95 Shank bassoon
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| }}
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| {{Box|font=120%|width=20%|GluR1 QDots diffusion rate|
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| * high - extrasynaptic
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| * medium - inactivated synapses
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| * low - active synapses
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| }}
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| {{Box|font=120%|width=20%|GluR1 QDots features|
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| * often passed through silenced synapses
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| * often moved from silenced to active synapse
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| * rarely (2 of 1700) moved from active to silenced synapse
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| * often (76.1%) exited inactive synapse (60 s period)
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| * rarely (21.4%) exited active synapse (60 s period)
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| }}
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| {{Box|font=120%|width=20%|GluR1 QDots diffusion rate after synaptic reversal|
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| * {{Nc}} at previously active synapses
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| * {{Nc}} at previously silenced synapses
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| }}
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| }}<!-- END ARTICLE -->
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| ==Important Studies==
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| {{ExpandBox|2003 Direct imaging of lateral movements of AMPA receptors inside synapses|
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| {{Article|Tardin, Cognet, Bats, Lounis, Choquet|2003|EMBO - [http://bradleymonk.com/media/Choquet5.pdf PDF]|12970178|Direct imaging of lateral movements of AMPA (GluR2) receptors inside synapses}}
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| ;Tested effects of glutamate and calcium influx on GluR2 diffusion.
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| *Anti-GluR2 antibodies labeled with Cy5 or Alexa-647
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| {{Box|width=20%|Glutamate|
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| ;synaptic
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| * {{Up}} GluR2
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| ;extrasynaptic
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| * {{Nc}} GluR2
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| }}
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| {{Box|width=20%|Calcium|
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| ;synaptic
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| * {{Dn}} GluR2 mobility
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| * {{Up}} GluR2 expression
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| ;extrasynaptic
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| * no observations
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| }}
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| ;Glutamate (100 uM) effect on GluR2
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| * {{Up}} diffusion rate
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| * {{Up}} (85%) endocytosis (within 15 min)
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| * {{Up}} (55%) diffusion within synapses
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| * {{Nc}} diffusion rate non-synaptic
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| * {{Dn}} (30%) completely immobile receptors
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| ;Calcium (induced) influx effect
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| * {{Dn}} (%) mobile GluR2
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| * {{Up}} (59%) GluR2 membrane expression
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| ;Calcium blocking (BAPTA) effect
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| * {{Up}} (%) mobile GluR2
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| }}<!-- END ARTICLE -->
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| {{ExpandBox|2004 Differential activity-dependent regulation of the lateral mobilities of AMPA and NMDA receptors|
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| {{Article|Groc L, Heine M, Cognet L, Brickley K, Stephenson FA, Lounis B, Choquet D.|2004|Nature Neuroscience - - [http://bradleymonk.com/media/Choquet6.pdf PDF]|15208630|Differential activity-dependent regulation of the lateral mobilities of AMPA and NMDA receptors}}
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| [[File:Choquet 2004b.png|thumb|left|500px|
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| * TTX - block neural activity <br>
| |
| * KCl - envoke neural activity <br>
| |
| * TPA - stimulate PKC
| |
| ]]{{Clear}}
| |
| | |
| {{Box|width=20%|KCl|
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| ;synaptic
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| * {{Up}}{{Nc}} GluR2
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| * {{Nc}} NMDAR
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| ;extrasynaptic
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| * {{Up}} GluR2
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| * {{Nc}} NMDAR
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| }} | | }} |
|
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| {{Box|width=20%|TTX|
| | <!-- |
| ;synaptic | | <html> |
| * {{Nc}} GluR2
| | <div style="position:absolute; top:-100px; left:-100px; width:100%; height:800px; z-index:10;"> |
| * {{Nc}} NMDAR
| | <iframe src="http://bradleymonk.com" width="100%" height="800px" scrolling="no" style="border:none"></iframe> |
| ;extrasynaptic | | <p><a href="http://bradleymonk.com" target="_blank">go to brads homepage</a></p> |
| * {{Dn}} GluR2
| | </div> |
| * {{Nc}} NMDAR
| | </html> |
| }}
| | --> |
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| {{Box|width=20%|TPA-PKC|
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| ;synaptic | |
| * {{Up}} GluR2
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| * {{Up}} NMDAR
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| ;extrasynaptic | |
| * {{Dn}} GluR2
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| * {{Up}} NMDAR
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| }}
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| }}<!-- END ARTICLE -->
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| {{ExpandBox|2007 Diffusional trapping of GluR1 AMPA receptors by input-specific synaptic activity|
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| {{Article|Ehlers, Heine, Groc, Lee, Choquet|2007|Neuron - [http://bradleymonk.com/media/Choquet2007B.pdf PDF]|17481397|Diffusional trapping of GluR1 AMPA receptors by input-specific synaptic activity}}
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| ;Silenced synapses (compared to active synapses)
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| * {{Dn}} (50%) GluR1
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| * {{Nc}} PSD-95
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| * {{Nc}} presynaptic VGLUT1
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| * {{Nc}} presynaptic bassoon
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| * {{Nc}} puncta size of PSD-95 Shank bassoon
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| ;GluR1 QDots diffusion rate
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| * high - extrasynaptic
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| * medium - inactivated synapses
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| * low - active synapses
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| ;GluR1 QDots features
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| * often passed through silenced synapses
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| * often moved from silenced to active synapse
| |
| * rarely (2 of 1700) moved from active to silenced synapse
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| * often (76.1%) exited inactive synapse (60 s period)
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| * rarely (21.4%) exited active synapse (60 s period)
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| | |
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| ;GluR1 QDots diffusion rate after synaptic reversal
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| * {{Nc}} at previously active synapses
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| * {{Nc}} at previously silenced synapses
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| [[File:Choquet2007B1.png|thumb|left|400px|
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| <b>Confinement of GluR1 in synapses</b><br>
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| * more confined at active synapses
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| ]]
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| }}<!-- END ARTICLE -->
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| {{ExpandBox|2007 Interaction between Stargazin and PSD-95 Regulates AMPA Receptor Surface Trafficking|
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| {{Article|Bats, Groc, Choquet|2007|Neuron - [http://bradleymonk.com/media/Choquet2007D.pdf PDF]|17329211|The Interaction between Stargazin and PSD-95 Regulates AMPA Receptor Surface Trafficking}}
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| ;Notes
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| * Quantum Dot
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| * FRAP
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| * Live hippocampal neurons
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| * exchange of [[AMPAR]] by lateral diffusion between extrasynaptic and synaptic sites mostly depends on the interaction of Stargazin with [[PSD-95]] and not upon the GluR2 [[AMPAR]] subunit C terminus.
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| * Disruption of interactions between Stargazin and [[PSD-95]] strongly increases [[AMPAR]] surface diffusion, preventing [[AMPAR]] accumulation at postsynaptic sites.
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| * AMPARs and Stargazin diffuse as complexes in and out synapses.
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| }}<!-- END ARTICLE -->
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| <pre> | |
| {{Article|Author|Year|Journal - [http://bradleymonk.com/media/Choquet1.pdf PDF]|15749166|Title}}
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| </pre>
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| __NOTOC__
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