User talk:Karishma Pratt: Difference between revisions

From bradwiki
Jump to navigation Jump to search
(→‎FRAP: new section)
No edit summary
Line 15: Line 15:
* Quantum Dot Tagging
* Quantum Dot Tagging
* Halotag (Promega): [[AMPAR]]-enzyme, QD-Halotag substrate
* Halotag (Promega): [[AMPAR]]-enzyme, QD-Halotag substrate
* AMPAR-streptavidin, QD-biotin
* streptavidin, QD-biotin
* AMPAR-FLAG, QD-anti-FLAG
* FLAG, QD-anti-FLAG
* AMPAR-biotin ligase recognition peptide, QD-biotin + biotin ligase (LuTing2013PLOSONE)
* biotin ligase recognition peptide
* AMPAR-peptide A, QD-peptide B, which binds peptide A (ZhangKodadek2000NatBiotech)
* unnatural amino acid azide, QD-propargyl
* AMPAR-unnatural amino acid azide, QD-propargyl (ChaterjeeSchultz2013PNAS)
}}
}}


Line 31: Line 30:
{{Clear}}
{{Clear}}


== FRAP ==


FRAP is a technique to do this...
==superecliptic phluorin (SEP)==
superecliptic phluorin (SEP) are used to do this...

Revision as of 12:14, 27 August 2013

Receptor Protein Tags

  • fluorescent proteins
  • superecliptic phluorin (SEP)
  • photoactivatable green fluorescent protein (PAGFP)

Protein Quantification

  • Western Blot
  • Quantitative Autoradiography

Molecular Tracking

  • Quantum Dot Tagging
  • Halotag (Promega): AMPAR-enzyme, QD-Halotag substrate
  • streptavidin, QD-biotin
  • FLAG, QD-anti-FLAG
  • biotin ligase recognition peptide
  • unnatural amino acid azide, QD-propargyl

Protein Interaction

  • FRET
  • Two Hybrid Screening
  • FRET or BRET
  • BiFC


superecliptic phluorin (SEP)

superecliptic phluorin (SEP) are used to do this...