File:Pollard2007SI2.mov: Difference between revisions

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({{Article|Fujiwara, Vavylonis, Pollard|2007|PNAS • [http://www.pnas.org/content/104/21/8827 FullText]|PMC1885587|Polymerization kinetics of ADP- and ADP-Pi-actin determined by fluorescence microscopy}} ;SI Movie 2. Time lapse (225´) movie of the elo...)
 
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Latest revision as of 01:38, 16 March 2015

SI Movie 2.

Time lapse (225´) movie of the elongation and depolymerization of ADP-actin filaments (30% Alexa label). The movie begins with filaments elongating in 5 mM Mg-ADP-actin in polymerization buffer. At the point indicated by "WASHOUT"-free actin monomers were washed out of the chamber with polymerization buffer with 0.2 mM ADP to allow depolymerization.

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current01:38, 16 March 2015 (1.21 MB)Bradley Monk (talk | contribs)
01:30, 16 March 2015 (1.27 MB)Bradley Monk (talk | contribs){{Article|Fujiwara, Vavylonis, Pollard|2007|PNAS • [http://www.pnas.org/content/104/21/8827 FullText]|PMC1885587|Polymerization kinetics of ADP- and ADP-Pi-actin determined by fluorescence microscopy}} ;SI Movie 2. Time lapse (225´) movie of the elo...

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