Cloning Protocol: Difference between revisions
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Bradley Monk (talk | contribs) (Created page with " Step 1: Phusion PCR Do a 200 uL reaction for the insert (2 PCR tubes with 100 uL) {| |- | 10 uM forward primer || 10 uL |- | 10 uM reverse primer || 10 uL |- | 2x phusion...") |
Bradley Monk (talk | contribs) No edit summary |
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{| | {| | ||
|- | |- | ||
| 10 uM forward primer || 10 uL | | 10 uM forward primer || || 10 uL | ||
|- | |- | ||
| 10 uM reverse primer || 10 uL | | 10 uM reverse primer || || 10 uL | ||
|- | |- | ||
| 2x phusion master mix (MM) || 100 uL | | 2x phusion master mix (MM) || || 100 uL | ||
|- | |- | ||
| 10 ng total template || 1 uL total | | 10 ng total template || || 1 uL total | ||
|- | |- | ||
| H2O up to 200 uL || variable - top to 200 uL | | H2O up to 200 uL || || variable - top to 200 uL | ||
|} | |} | ||
Revision as of 10:02, 11 January 2016
Step 1: Phusion PCR
Do a 200 uL reaction for the insert (2 PCR tubes with 100 uL)
| 10 uM forward primer | 10 uL | |
| 10 uM reverse primer | 10 uL | |
| 2x phusion master mix (MM) | 100 uL | |
| 10 ng total template | 1 uL total | |
| H2O up to 200 uL | variable - top to 200 uL |