Malinow

• Molecular Methods

Notes

• FLASH technology
• Bredt
• minisog - gfp
• Acidic basic polypeptide recognition sequences
• Talk with nanotech group about various ways to conj. Qdots
• Nichol and England - couple Qdot to AMPAR agonist
• Have simulation be a competitive model where AMPARs are competing during LTP
• Quantitative review on synaptic numbers (Sheng)

PALM STORM

There are two major groups of methods for functional super-resolution microscopy:

1. Deterministic super-resolution: The most commonly used emitters in biological microscopy, fluorophores, show a nonlinear response to excitation, and this nonlinear response can be exploited to enhance resolution. These methods include STED, GSD, RESOLFT and SSIM.

2. Stochastical super-resolution PALM STORM: The chemical complexity of many molecular light sources gives them a complex temporal behaviour, which can be used to make several close-by fluorophores emit light at separate times and thereby become resolvable in time. These methods include SOFI and all single-molecule localization methods (SMLM) such as SPDM, SPDMphymod, PALM, FPALM, STORM and dSTORM.

NRSA

• Dominant negative PSD95