Brad: Difference between revisions
Jump to navigation
Jump to search
Bradley Monk (talk | contribs) No edit summary |
Bradley Monk (talk | contribs) |
||
| Line 62: | Line 62: | ||
{{Article|Ehlers, Heine, Groc, Lee, Choquet|2007|Neuron - [http://bradleymonk.com/media/Choquet2007B.pdf PDF]|17481397|Diffusional trapping of GluR1 AMPA receptors by input-specific synaptic activity}} | {{Article|Ehlers, Heine, Groc, Lee, Choquet|2007|Neuron - [http://bradleymonk.com/media/Choquet2007B.pdf PDF]|17481397|Diffusional trapping of GluR1 AMPA receptors by input-specific synaptic activity}} | ||
;Silenced synapses (compared to active synapses) | |||
; | |||
* {{Dn}} (50%) GluR1 | * {{Dn}} (50%) GluR1 | ||
* {{Nc}} PSD-95 | * {{Nc}} PSD-95 | ||
| Line 69: | Line 68: | ||
* {{Nc}} presynaptic bassoon | * {{Nc}} presynaptic bassoon | ||
* {{Nc}} puncta size of PSD-95 Shank bassoon | * {{Nc}} puncta size of PSD-95 Shank bassoon | ||
;GluR1 QDots diffusion rate | ;GluR1 QDots diffusion rate | ||
| Line 74: | Line 74: | ||
* medium - inactivated synapses | * medium - inactivated synapses | ||
* low - active synapses | * low - active synapses | ||
;GluR1 QDots features | ;GluR1 QDots features | ||
| Line 81: | Line 82: | ||
* often (76.1%) exited inactive synapse (60 s period) | * often (76.1%) exited inactive synapse (60 s period) | ||
* rarely (21.4%) exited active synapse (60 s period) | * rarely (21.4%) exited active synapse (60 s period) | ||
;GluR1 QDots diffusion rate after synaptic reversal | ;GluR1 QDots diffusion rate after synaptic reversal | ||
| Line 89: | Line 91: | ||
[[File:Choquet2007B1.png|thumb|left|400px| | [[File:Choquet2007B1.png|thumb|left|400px| | ||
<b>Confinement of GluR1 in synapses</b><br> | <b>Confinement of GluR1 in synapses</b><br> | ||
* more confined at active | * more confined at active synapses | ||
]] | ]] | ||
Revision as of 20:20, 9 July 2013
| Malinow | Molecular Methods | Quantum Dots | Choquet | AMPAR |
Study Timeline - PubMed
Summary
2003 Direct imaging of lateral movements of AMPA receptors inside synapses
Tardin, Cognet, Bats, Lounis, Choquet • 2003 • EMBO - PDF
- Tested effects of glutamate and calcium influx on GluR2 diffusion.
- Anti-GluR2 antibodies labeled with Cy5 or Alexa-647
- Glutamate (100 uM) effect on GluR2
- ↑ diffusion rate
- ↑ (85%) endocytosis (within 15 min)
- ↑ (55%) diffusion within synapses
- ↔ diffusion rate non-synaptic
- ↓ (30%) completely immobile receptors
- Calcium (induced) influx effect
- ↓ (%) mobile GluR2
- ↑ (59%) GluR2 membrane expression
- Calcium blocking (BAPTA) effect
- ↑ (%) mobile GluR2
2004 Differential activity-dependent regulation of the lateral mobilities of AMPA and NMDA receptors
Groc L, Heine M, Cognet L, Brickley K, Stephenson FA, Lounis B, Choquet D. • 2004 • Nature Neuroscience - - PDF
- GluR2 vs. NMDAR-(NR1) diffusion
- extrasynaptic: GluR2 > NMDAR (4x)
- synaptic: GluR2 ↔ NMDAR
- synaptic > extrasynaptic (2x ??)
- KCl Neural Stimulation
- ↑ (5x) extrasynaptic diffusion rate GluR2
- ↔ (%) synaptic diffusion rate GluR2
- PKC activity (stim by TPA)
- ↑ extrasynaptic diffusion rate GluR2 & NMDAR
- ↑ synaptic diffusion rate GluR2 & NMDAR
* KCl - envoke neural activity
* TPA - stimulate PKC
2007 Diffusional trapping of GluR1 AMPA receptors by input-specific synaptic activity
Ehlers, Heine, Groc, Lee, Choquet • 2007 • Neuron - PDF
- Silenced synapses (compared to active synapses)
- ↓ (50%) GluR1
- ↔ PSD-95
- ↔ presynaptic VGLUT1
- ↔ presynaptic bassoon
- ↔ puncta size of PSD-95 Shank bassoon
- GluR1 QDots diffusion rate
- high - extrasynaptic
- medium - inactivated synapses
- low - active synapses
- GluR1 QDots features
- often passed through silenced synapses
- often moved from silenced to active synapse
- rarely (2 of 1700) moved from active to silenced synapse
- often (76.1%) exited inactive synapse (60 s period)
- rarely (21.4%) exited active synapse (60 s period)
- GluR1 QDots diffusion rate after synaptic reversal
- ↔ at previously active synapses
- ↔ at previously silenced synapses
* more confined at active synapses
2007 Interaction between Stargazin and PSD-95 Regulates AMPA Receptor Surface Trafficking
Bats, Groc, Choquet • 2007 • Neuron - PDF
- Notes
- Quantum Dot
- FRAP
- Live hippocampal neurons
- exchange of AMPAR by lateral diffusion between extrasynaptic and synaptic sites mostly depends on the interaction of Stargazin with PSD-95 and not upon the GluR2 AMPAR subunit C terminus.
- Disruption of interactions between Stargazin and PSD-95 strongly increases AMPAR surface diffusion, preventing AMPAR accumulation at postsynaptic sites.
- AMPARs and Stargazin diffuse as complexes in and out synapses.
{{Article|Author|Year|Journal - [http://bradleymonk.com/media/Choquet1.pdf PDF]|15749166|Title}}