User talk:Karishma Pratt: Difference between revisions

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{{Box|Cell Culture|width=45%|
{{Box|Cell Culture|width=45%|
* Rat Hippocampus Neurons E18
* [http://www.lifetechnologies.com/us/en/home/references/protocols/neurobiology/neurobiology-protocols/isolation-culture-and-characterization-of-cortical-and-hippocampal-neurons.html Rat Hippocampus Neurons E18]
}}
}}



Revision as of 14:49, 13 September 2013

Receptor Protein Tags

Protein Quantification

  • Western Blot
  • Quantitative Autoradiography
  • FRAP

Molecular Tracking

  • Quantum Dot Tagging
  • Halotag (Promega): AMPAR-enzyme, QD-Halotag substrate
  • streptavidin, QD-biotin
  • FLAG, QD-anti-FLAG
  • biotin ligase recognition peptide
  • unnatural amino acid azide, QD-propargyl

Protein Interaction

  • FRET
  • Two Hybrid Screening
  • FRET or BRET
  • BiFC

Cell Culture


superecliptic phluorin SEP

superecliptic phluorin (SEP) are used to do this...