Glutamate Uncaging: Difference between revisions
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===MNI-Glutamate=== | ===MNI-Glutamate=== | ||
2-photon uncaging of MNI-glutamate has been used successfully to functionally map synaptic receptors <ref name="Araya2006"></ref><ref name="Carter2004"></ref><ref name="Gasparini2006"></ref><ref name="Matsuzaki2004"></ref><ref name="Sobczyk2005"></ref>, activate individual spines, and individual neurons. | 2-photon uncaging of MNI-glutamate has been used successfully to functionally map synaptic receptors <ref name="Araya2006"></ref><ref name="Carter2004"></ref><ref name="Gasparini2006"></ref><ref name="Matsuzaki2004"></ref><ref name="Sobczyk2005"></ref>, activate individual spines, and individual neurons. | ||
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===CNB-glutamate=== | ===CNB-glutamate=== | ||
CNB-glutamate | CNB-glutamate | ||
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===L-glutamic acid=== | ===L-glutamic acid=== | ||
L-glutamic acid α(4,5-dimethoxy-2-nitrobenzyl) ester (Callaway, Katz, 1993) | L-glutamic acid α(4,5-dimethoxy-2-nitrobenzyl) ester (Callaway, Katz, 1993) | ||
Revision as of 20:12, 28 November 2015
One of the first experiments uncaging glutamate on dendrites was done by Kandler on CA1 pyramidal neurons in slices. They reported the long-term depression of glutamate responses after the pairing of uncaging with depolarization of the neuron. A similar result was obtained with bursts of uncaging by Dodt; these authors used an infrared-guided laser stimulation system to uncage glutamate on the apical dendrites of layer 5 neocortical pyramidal neurons.
Uncaging Compounds
RuBi-Glutamate
- caged-glutamate compound
- based on ruthenium photochemistry
- excited with visible wavelengths
- releases glutamate after 1-photon or 2-photon excitation.
- high quantum efficiency
- can be used at low concentrations,
- partly avoids blockade of GABAergic transmission
- 2-photon uncaging of RuBi-Glutamate has a high spatial resolution and generates excitatory responses in individual dendritic spines with physiological kinetics.
- With laser beam multiplexing, two-photon RuBi-Glutamate uncaging can also be used to depolarize and fire pyramidal neurons with single-cell resolution.
- RuBi-Glutamate enables the photoactivation of neuronal dendrites and circuits with visible or two-photon light sources, achieving single cell, or even single spine, precision.
ruthenium-bipyridine complexes can be used as caging compounds. ruthenium is a transition metal with versatile chemistry. polypyridines of ruthenium photorelease entire ligands in a heterolytic fashion, by means of a widely known mechanism in which the initial photoexcited state quickly evolves into a dissociative state, so the photorelease is therefore clean and fast.
- Pros: RuBi-Glutamate
- minimal antagonistic effects on GABAergic transmission
- Cons: RuBi-Glutamate
- not much prior literature to demo the effects
MNI-Glutamate
2-photon uncaging of MNI-glutamate has been used successfully to functionally map synaptic receptors [1][2][3][4][5], activate individual spines, and individual neurons.
- Pros: MNI-glutamate
- lots of prior literature to demo the effects
- antagonist of GABAergic transmission at high concentrations
- Cons: MNI-glutamate
- needs to be applied to the tissue at relatively high (mM) concentrations for effective 2-photon uncaging.
- antagonist to GABAergic transmission so can't be used to study GABAergic neurons
CNB-glutamate
CNB-glutamate
L-glutamic acid
L-glutamic acid α(4,5-dimethoxy-2-nitrobenzyl) ester (Callaway, Katz, 1993)
REFERENCES
<references>
- ↑ 1.0 1.1 Araya Yuste 2006 The spine neck filters membrane potentials
- ↑ 2.0 2.1 Carter Sabatini 2004 State-dependent calcium signaling in dendritic spines of striatal medium spiny neurons
- ↑ 3.0 3.1 Gasparini Magee 2006 State dependent dendritic computation in hippocampal CA1 pyramidal neurons
- ↑ 4.0 4.1 Matsuzaki Kasai 2001 Dendritic spine geometry is critical for AMPA receptor expression in hippocampal CA1 pyramidal neurons
- ↑ 5.0 5.1 Sobczyk Svoboda 2005 NMDA receptor subunitdependent Ca2 signaling in individual hippocampal dendritic spines
- Salierno Marceca Peterka Yuste Etchenique 2010 fast ruthenium polypyridine cage complex photoreleases glutamate with visible or IR light in one and two photon regimes J Inorg Biochem
- Callaway Katz 1993 Photostimulation using caged glutamate reveals functional circuitry in living brain slices
- Callaway Yuste 2002 Stimulating neurons with light
- Frick Dodt 2001 Glutamate receptors form hot spots on apical dendrites of neocortical pyramidal neurons
- Pettit Augustine 1997 Chemical twophoton uncaging: a novel approach to mapping glutamate receptors
- Shepherd Svoboda 2003 Circuit analysis of experience-dependent plasticity in the developing rat barrel cortex
- Wieboldt Hess 1994 Photolabile precursors of glutamate: synthesis, photochemical properties, and activation of glutamate receptors on a microsecond time scale.
- Yoshimura Callaway 2005 Excitatory cortical neurons form fine-scale functional networks.