Receptor Protein Tags
- fluorescent proteins
- superecliptic phluorin (SEP)
- photoactivatable green fluorescent protein (PAGFP)
Protein Quantification
- Western Blot
- Quantitative Autoradiography
Molecular Tracking
- Quantum Dot Tagging
- Halotag (Promega): AMPAR-enzyme, QD-Halotag substrate
- AMPAR-streptavidin, QD-biotin
- AMPAR-FLAG, QD-anti-FLAG
- AMPAR-biotin ligase recognition peptide, QD-biotin + biotin ligase (LuTing2013PLOSONE)
- AMPAR-peptide A, QD-peptide B, which binds peptide A (ZhangKodadek2000NatBiotech)
- AMPAR-unnatural amino acid azide, QD-propargyl (ChaterjeeSchultz2013PNAS)
Protein Interaction
- FRET
- Two Hybrid Screening
- FRET or BRET
- BiFC
FRAP
FRAP is a technique to do this...