AMPA Receptor

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Binding Assay 2-amino-3-(3-hydroxy-5-methyl-4-isoxazolyl) propionic acid (AMPA) receptors
PROCEDURES
Radioligand Pre-Incubation Incubation Wash
3H-AMPA
15 nM		 50 mM Tris-HCl
100 mM KCl
pH 7.4
10 min		 15 nM 3H-AMPA
Pre-Incubation Buffer
60 min		 Pre-Incubation Buffer
10 min x2 baths
1 dip diH2O
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