Cloning Protocol

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Step 1: Phusion PCR

Do a 200 uL reaction for the insert (2 PCR tubes with 100 uL)

10 uM forward primer		10 uL
10 uM reverse primer		10 uL
2x phusion master mix (MM)		100 uL
10 ng total template		1 uL total
H2O up to 200 uL		variable - top to 200 uL

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