Cloning Protocol

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Step 1: Phusion PCR

Do a 200 uL reaction for the insert (2 PCR tubes with 100 uL)

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10 uM forward primer     10 uL
10 uM reverse primer 10 uL
2x phusion master mix (MM) 100 uL
10 ng total template 1 uL total
H2O up to 200 uL variable - top to 200 uL
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