Summary ▼ 2003 Direct imaging of lateral movements of AMPA receptors inside synapses
Tardin, Cognet, Bats, Lounis, Choquet
• 2003 • EMBO - PDF
Tested effects of glutamate and calcium influx on GluR2 diffusion. Anti-GluR2 antibodies labeled with Cy5 or Alexa-647
Glutamate (100 uM) effect on GluR2 ↑ ↑ ↑ ↔ ↓
Calcium (induced) influx effect ↓ ↑
Calcium blocking (BAPTA) effect
▼ 2004 Differential activity-dependent regulation of the lateral mobilities of AMPA and NMDA receptors
Groc L, Heine M, Cognet L, Brickley K, Stephenson FA, Lounis B, Choquet D.
• 2004 • Nature Neuroscience - - PDF
GluR2 vs. NMDAR-(NR1) diffusion extrasynaptic: GluR2 > NMDAR (4x)
synaptic: GluR2 ↔
synaptic > extrasynaptic (2x ??) KCl Neural Stimulation
↑ ↔ PKC activity (stim by TPA)
↑ ↑ Error creating thumbnail: File missing * TTX - block neural activity
▼ 2007 Diffusional trapping of GluR1 AMPA receptors by input-specific synaptic activity
Ehlers, Heine, Groc, Lee, Choquet
• 2007 • Neuron - PDF
Silenced synapses (compared to active synapses) ↓ ↔ ↔ ↔ ↔
GluR1 QDots diffusion rate high - extrasynaptic
medium - inactivated synapses
low - active synapses
GluR1 QDots features often passed through silenced synapses
often moved from silenced to active synapse
rarely (2 of 1700) moved from active to silenced synapse
often (76.1%) exited inactive synapse (60 s period)
rarely (21.4%) exited active synapse (60 s period)
GluR1 QDots diffusion rate after synaptic reversal ↔ ↔
Error creating thumbnail: File missing Confinement of GluR1 in synapses
▼ 2007 Interaction between Stargazin and PSD-95 Regulates AMPA Receptor Surface Trafficking
Bats, Groc, Choquet
• 2007 • Neuron - PDF
Notes Quantum Dot
FRAP
Live hippocampal neurons
exchange of AMPAR by lateral diffusion between extrasynaptic and synaptic sites mostly depends on the interaction of Stargazin with PSD-95 and not upon the GluR2 AMPAR subunit C terminus.
Disruption of interactions between Stargazin and PSD-95 strongly increases AMPAR surface diffusion, preventing AMPAR accumulation at postsynaptic sites.
AMPARs and Stargazin diffuse as complexes in and out synapses.
{{Article|Author|Year|Journal - [http://bradleymonk.com/media/Choquet1.pdf PDF]|15749166|Title}}
