Brad
| Malinow | Molecular Methods | Quantum Dots | Choquet | AMPAR |
Overall Summary
Complete Picture
* KCl - envoke neural activity
* TPA - stimulate PKC
KCl
TTX
TPA-PKC
Glutamate
Calcium
Important Studies
2003 Direct imaging of lateral movements of AMPA receptors inside synapses
Tardin, Cognet, Bats, Lounis, Choquet • 2003 • EMBO - PDF
- Tested effects of glutamate and calcium influx on GluR2 diffusion.
- Anti-GluR2 antibodies labeled with Cy5 or Alexa-647
Glutamate
Calcium
- Glutamate (100 uM) effect on GluR2
- ↑ diffusion rate
- ↑ (85%) endocytosis (within 15 min)
- ↑ (55%) diffusion within synapses
- ↔ diffusion rate non-synaptic
- ↓ (30%) completely immobile receptors
- Calcium (induced) influx effect
- ↓ (%) mobile GluR2
- ↑ (59%) GluR2 membrane expression
- Calcium blocking (BAPTA) effect
- ↑ (%) mobile GluR2
2004 Differential activity-dependent regulation of the lateral mobilities of AMPA and NMDA receptors
Groc L, Heine M, Cognet L, Brickley K, Stephenson FA, Lounis B, Choquet D. • 2004 • Nature Neuroscience - - PDF
* KCl - envoke neural activity
* TPA - stimulate PKC
KCl
TTX
TPA-PKC
2007 Diffusional trapping of GluR1 AMPA receptors by input-specific synaptic activity
Ehlers, Heine, Groc, Lee, Choquet • 2007 • Neuron - PDF
- Silenced synapses (compared to active synapses)
- ↓ (50%) GluR1
- ↔ PSD-95
- ↔ presynaptic VGLUT1
- ↔ presynaptic bassoon
- ↔ puncta size of PSD-95 Shank bassoon
- GluR1 QDots diffusion rate
- high - extrasynaptic
- medium - inactivated synapses
- low - active synapses
- GluR1 QDots features
- often passed through silenced synapses
- often moved from silenced to active synapse
- rarely (2 of 1700) moved from active to silenced synapse
- often (76.1%) exited inactive synapse (60 s period)
- rarely (21.4%) exited active synapse (60 s period)
- GluR1 QDots diffusion rate after synaptic reversal
- ↔ at previously active synapses
- ↔ at previously silenced synapses
* more confined at active synapses
2007 Interaction between Stargazin and PSD-95 Regulates AMPA Receptor Surface Trafficking
Bats, Groc, Choquet • 2007 • Neuron - PDF
- Notes
- Quantum Dot
- FRAP
- Live hippocampal neurons
- exchange of AMPAR by lateral diffusion between extrasynaptic and synaptic sites mostly depends on the interaction of Stargazin with PSD-95 and not upon the GluR2 AMPAR subunit C terminus.
- Disruption of interactions between Stargazin and PSD-95 strongly increases AMPAR surface diffusion, preventing AMPAR accumulation at postsynaptic sites.
- AMPARs and Stargazin diffuse as complexes in and out synapses.
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