Brad
| Malinow | Molecular Methods | Quantum Dots | Choquet | AMPAR |
Overall Summary
Complete Picture
* KCl - envoke neural activity
* TPA - stimulate PKC
- 2004
KCl
TTX
TPA-PKC
- 2003
Glutamate
Calcium
- 2007
Silenced (vs active) synapses
GluR1 QDots diffusion rate
GluR1 QDots features
GluR1 QDots diffusion rate after synaptic reversal
Important Studies
2003 Direct imaging of lateral movements of AMPA receptors inside synapses
Tardin, Cognet, Bats, Lounis, Choquet • 2003 • EMBO - PDF
- Tested effects of glutamate and calcium influx on GluR2 diffusion.
- Anti-GluR2 antibodies labeled with Cy5 or Alexa-647
Glutamate
Calcium
- Glutamate (100 uM) effect on GluR2
- ↑ diffusion rate
- ↑ (85%) endocytosis (within 15 min)
- ↑ (55%) diffusion within synapses
- ↔ diffusion rate non-synaptic
- ↓ (30%) completely immobile receptors
- Calcium (induced) influx effect
- ↓ (%) mobile GluR2
- ↑ (59%) GluR2 membrane expression
- Calcium blocking (BAPTA) effect
- ↑ (%) mobile GluR2
2004 Differential activity-dependent regulation of the lateral mobilities of AMPA and NMDA receptors
Groc L, Heine M, Cognet L, Brickley K, Stephenson FA, Lounis B, Choquet D. • 2004 • Nature Neuroscience - - PDF
* KCl - envoke neural activity
* TPA - stimulate PKC
KCl
TTX
TPA-PKC
2007 Diffusional trapping of GluR1 AMPA receptors by input-specific synaptic activity
Ehlers, Heine, Groc, Lee, Choquet • 2007 • Neuron - PDF
- Silenced synapses (compared to active synapses)
- ↓ (50%) GluR1
- ↔ PSD-95
- ↔ presynaptic VGLUT1
- ↔ presynaptic bassoon
- ↔ puncta size of PSD-95 Shank bassoon
- GluR1 QDots diffusion rate
- high - extrasynaptic
- medium - inactivated synapses
- low - active synapses
- GluR1 QDots features
- often passed through silenced synapses
- often moved from silenced to active synapse
- rarely (2 of 1700) moved from active to silenced synapse
- often (76.1%) exited inactive synapse (60 s period)
- rarely (21.4%) exited active synapse (60 s period)
- GluR1 QDots diffusion rate after synaptic reversal
- ↔ at previously active synapses
- ↔ at previously silenced synapses
* more confined at active synapses
2007 Interaction between Stargazin and PSD-95 Regulates AMPA Receptor Surface Trafficking
Bats, Groc, Choquet • 2007 • Neuron - PDF
- Notes
- Quantum Dot
- FRAP
- Live hippocampal neurons
- exchange of AMPAR by lateral diffusion between extrasynaptic and synaptic sites mostly depends on the interaction of Stargazin with PSD-95 and not upon the GluR2 AMPAR subunit C terminus.
- Disruption of interactions between Stargazin and PSD-95 strongly increases AMPAR surface diffusion, preventing AMPAR accumulation at postsynaptic sites.
- AMPARs and Stargazin diffuse as complexes in and out synapses.
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