STARShiP

Expand to view experiment animation

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%%
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%					SPINE RADIAL DISTANCE
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%=================================================
for reN = 1:loops
%=================================================

SGr = reSGr{reN};

%--------------------------------------
% Make concentric circle annulus
%--------------------------------------
S1r = round(S1rad);
Qhr = S1r/2;
Qx = linspace(0,S1r,6);
QxL = Qx - Qhr;
yL = sqrt(Qhr.^2 - QxL.^2);
Qr = sqrt(Qx.^2 + yL.^2);	% Concentric circles

Quantitative Review

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Harris Website

The Size of Dendrites

adapted from Sheng and Hoogenraad (2007) ^FIG:{{#info: Error creating thumbnail: File missing{{{2}}} CLICK AWAY FROM IMAGE TO CLOSE }}

Dendrite: 1–10 spines per 10 μm
Spines: 0.5–2 μm in length
PSD: 100 - 300 nm diameter
PSD95: within 12 nm of surface

adapted from Harris ^FIG:{{#info: {{{2}}} CLICK AWAY FROM IMAGE TO CLOSE }}

proximal dendrite diameter: 1 - 3 µm
distal dendrite diameter: 0.2 - 2 µm
dendrite length: 2000 - 9000 µm
dendrite tip to soma: 100 - 200 µm
dendrites at soma: 1 - 5
dendrite branches (granual): 10 - 30
dendrite branches (purkinje): 400-500

Particle Counts

adapted from Sheng and Hoogenraad (2007) ^FIG:{{#info: Error creating thumbnail: File missing{{{2}}} CLICK AWAY FROM IMAGE TO CLOSE }}

PSD: 10,000 proteins (or 100 copies of 100 proteins)
CaMKIIα: 7.4%
CaMKIIβ: 1.3%
SynGap: 2.1 pmol/20 μg
NMDAR: 20 proteins
AMPAR: 15 proteins
GluR: 60 subunits, 15 tetramers, 80% or 12 GluR1/GluR2 heteromers
PSD95: within 12 nm of surface

Diffusion Rates

from Choquet 2010 ^FIG:{{#info: {{{2}}} CLICK AWAY FROM IMAGE TO CLOSE }}

extrasynaptic: 0.1 µm²⁄s
synaptic: 0.05 µm²⁄s
synaptic after glu/gly: 0.01 µm²⁄s

Images

From Sheng and Hoogenraad 2007

Spine morphology ^FIG:{{#info: Error creating thumbnail: File missing{{{2}}} CLICK AWAY FROM IMAGE TO CLOSE }}

From Harris KM and Weinberg 2012

Spine morphology ^FIG:{{#info: 3D reconstruction of a proximal CA3 pyramidal cell dendrite (blue) and a large mossy fiber bouton (translucent yellow). The cut-away in C2 shows synapses (red) onto multiple dendritic spines, some of which are highly branched. The bouton also forms nonsynaptic cell adhesion junctions (fuchsia). CLICK AWAY FROM IMAGE TO CLOSE }}
Hippocampal dendrite ^FIG:{{#info: {{{2}}} CLICK AWAY FROM IMAGE TO CLOSE }}

Choquet 2007 Real Time Receptor Diffusion

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Choquet 2007 Real Time Receptor Diffusion Analysis

The video represents a 10µm × 10µm section scaled to a 535px × 535px video.
- 1_µm : 53.5_px
The analysis below documents one instance of Qdot diffusion, between the 6s-7s time points.
This instance was chosen because of the clarity of motion and no Qdot flicker.
The Qdot (center) moves from pixel location (X:291, Y:302) at 6.78s to (X:319, Y346) at 6.98s
- That is a distance of 52.2px in 200ms
- Qdot velocity: Q_v ≈ 1_µm ⁄ 200_ms
- Note this diffusion rate of 5µm/s is 10-fold higher than the median diffusion rate reported above.
- An upper bound of 5µm/s means that receptors can move between synapses in fractions of a second.