File:MacGillavry Blanpied 2013 Fig3.png

Figure 3

Figure 3. Scaffold Organization in Subsynaptic Clusters Enriched in AMPARs (A) Examples of PSDs resolved with PALM for mEos2-tagged shrPSD-95, GKAP, shrShank3, and Homer1c. Scale bar represents 200 nm. (B) Relative frequency of PSDs with 0, 1, or more clusters. (C and D) Cumulative frequency distribution (C) and mean of cluster area (D) for different scaffold molecules. (E and F) Examples of twocolor single-molecule imaging for shrPSD-95- mEos2 and GluA2 (E) or GluN2B (F). Scale bar represents 200 nm. (G) Relative enrichment of PSD-95, GluA2, GluN1, GluN2A, and GluN2B localizations inside and outside of subsynaptic PSD- 95 clusters. Alexa 647 localizations within the PSD defined by PSD-95-mEos2 (polygonal) were extracted, and the densities in and out of the subdomain (circle) were compared. ***p < 0.001, *p < 0.05, two-way ANOVA with Bonferroni post hoc test. (H) Examples of PSDs resolved with PALM in cerulean3 or GluA1,2 C-tail expressing neurons. (I and J) Cumulative frequency distribution and mean of cluster area for control (cer3) and GluA1,2 C-tail (tails) expressing neurons. **p < 0.01, different from control, Kolmogorov-Smirnov test. All error bars represent SEM.