File:MacGillavry Blanpied 2013 Fig2.png

Figure 2

Figure 2. Heterogeneous Distribution of Scaffold Molecules within the Postsynaptic Density (A and B) Single-molecule localization of PSD-95- Eos2. Individual molecules were color coded according to their local density (B), and the number of molecules within a radius five times the average nearest neighbor distance within the PSD (blue and red circles). Scale bar represents 100 nm. (C) Homogenous distribution (right) generated by randomly sampling equal numbers of localizations as observed (left). Scale bar represents 100 nm. (D) Mean pair-correlation function of the PSD in (C) for the measured particle locations (blue) and for the simulated locations (red). Shaded areas represent 99% confidence intervals calculated from the randomized ensembles, showing significant departures from homogeneity. (E) Example of a time series of shrPSD-95-mEos2 local density plots, revealing time-dependent variation in the distribution of PSD-95 molecules within the PSD. Scale bar represents 100 nm.