Cloning Protocol

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Step 1: Phusion PCR

Do a 200 uL reaction for the insert (2 PCR tubes with 100 uL)


10 uM forward primer 10 uL
10 uM reverse primer 10 uL
2x phusion master mix (MM) 100 uL
10 ng total template 1 uL total
H2O up to 200 uL variable - top to 200 uL
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