Category:Neuroscience Methods: Difference between revisions
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{{Box|Receptor Protein Tags| | {{Box|Receptor Protein Tags|width=45%| | ||
* fluorescent proteins | * fluorescent proteins | ||
* superecliptic phluorin (SEP) | * superecliptic phluorin (SEP) | ||
* photoactivatable green fluorescent protein (PAGFP) | * photoactivatable green fluorescent protein (PAGFP) | ||
}} | }} | ||
{{Box|Protein Quantification|width=45%| | |||
* Western Blot | |||
* Quantitative Autoradiography | |||
}} | |||
{{Clear}} | |||
{{Box|Molecular Tracking|width=45%| | |||
* Quantum Dot Tagging | |||
:'''Large [[AMPAR]] N-terminal addition''' | |||
:* Halotag (Promega): [[AMPAR]]-enzyme, QD-Halotag substrate | |||
:* [[AMPAR]]-streptavidin, QD-biotin | |||
:'''Small [[AMPAR]] N-terminal addition''' | |||
:* [[AMPAR]]-FLAG, QD-anti-FLAG | |||
:* [[AMPAR]]-biotin ligase recognition peptide, QD-biotin + biotin ligase (LuTing2013PLOSONE) | |||
:* [[AMPAR]]-peptide A, QD-peptide B, which binds peptide A (ZhangKodadek2000NatBiotech) | |||
:* [[AMPAR]]-unnatural amino acid azide, QD-propargyl (ChaterjeeSchultz2013PNAS) | |||
}} | |||
{{Box|Protein Interaction|width=45%| | |||
* FRET | |||
* Two Hybrid Screening | |||
:* [http://en.wikipedia.org/wiki/F%C3%B6rster_resonance_energy_transfer FRET] or BRET | |||
:* [http://en.wikipedia.org/wiki/Bimolecular_fluorescence_complementation BiFC] | |||
}} | |||
{{Clear}} | |||
{{Box|Cell Morphology|width=45%| | |||
* Optical Defractionation | |||
:* [http://en.wikipedia.org/wiki/Super_resolution_microscopy#Photoactivated_localization_microscopy_.28PALM.29_and_FPALM PALM STORM] | |||
:* [http://en.wikipedia.org/wiki/Fluorescence-lifetime_imaging_microscopy Fluorescence-lifetime imaging microscopy or FLIM] | |||
:* [http://en.wikipedia.org/wiki/Two-photon_excitation_microscopy Two-photon Microscopy] | |||
}} | |||
{{Box|Controlling Neural Activity|width=45%| | |||
* Optogenetics | |||
* Protein Uncaging | |||
}} | |||
{{Box|Gene Related Assays|width=45%| | |||
* TET/DOX transcription regulation | |||
* CRE/LOX | |||
}} | |||
{{Clear}} | |||
Latest revision as of 11:33, 27 August 2013
Receptor Protein Tags
Protein Quantification
Molecular Tracking
Protein Interaction
Cell Morphology
Controlling Neural Activity
Gene Related Assays
Pages in category "Neuroscience Methods"
The following 5 pages are in this category, out of 5 total.