Category:Neuroscience Methods: Difference between revisions

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{{Box|Receptor Protein Tags|width=45%|
{{Box|Protein Quantification|width=45%|
* fluorescent proteins
* Western Blot
* superecliptic phluorin (SEP)
* Quantitative Autoradiography
* photoactivatable green fluorescent protein (PAGFP)
}}
 
{{Clear}}
 
{{Box|Molecular Tracking|width=45%|
* Quantum Dot Tagging
:'''Large [[AMPAR]] N-terminal addition'''
:* Halotag (Promega): [[AMPAR]]-enzyme, QD-Halotag substrate
:* [[AMPAR]]-streptavidin, QD-biotin
 
:'''Small [[AMPAR]] N-terminal addition'''
:* [[AMPAR]]-FLAG, QD-anti-FLAG
:* [[AMPAR]]-biotin ligase recognition peptide, QD-biotin + biotin ligase (LuTing2013PLOSONE)
:* [[AMPAR]]-peptide A, QD-peptide B, which binds peptide A (ZhangKodadek2000NatBiotech)
:* [[AMPAR]]-unnatural amino acid azide, QD-propargyl (ChaterjeeSchultz2013PNAS)
}}
 
{{Box|Protein Interaction|width=45%|
* FRET
* Two Hybrid Screening
:* [http://en.wikipedia.org/wiki/F%C3%B6rster_resonance_energy_transfer FRET] or BRET
:* [http://en.wikipedia.org/wiki/Bimolecular_fluorescence_complementation BiFC]
}}
 
{{Clear}}
 
{{Box|Cell Morphology|width=45%|
* Optical Defractionation
:* [http://en.wikipedia.org/wiki/Super_resolution_microscopy#Photoactivated_localization_microscopy_.28PALM.29_and_FPALM PALM STORM]
:* [http://en.wikipedia.org/wiki/Fluorescence-lifetime_imaging_microscopy Fluorescence-lifetime imaging microscopy or FLIM]
:* [http://en.wikipedia.org/wiki/Two-photon_excitation_microscopy Two-photon Microscopy]
}}
 
{{Box|Controlling Neural Activity|width=45%|
* Optogenetics
* Protein Uncaging
}}
 
{{Box|Gene Related Assays|width=45%|
* TET/DOX transcription regulation
* CRE/LOX
}}
}}
{{Clear}}
{{Clear}}

Latest revision as of 11:33, 27 August 2013

Receptor Protein Tags

  • fluorescent proteins
  • superecliptic phluorin (SEP)
  • photoactivatable green fluorescent protein (PAGFP)

Protein Quantification

  • Western Blot
  • Quantitative Autoradiography

Molecular Tracking

  • Quantum Dot Tagging
Large AMPAR N-terminal addition
  • Halotag (Promega): AMPAR-enzyme, QD-Halotag substrate
  • AMPAR-streptavidin, QD-biotin
Small AMPAR N-terminal addition
  • AMPAR-FLAG, QD-anti-FLAG
  • AMPAR-biotin ligase recognition peptide, QD-biotin + biotin ligase (LuTing2013PLOSONE)
  • AMPAR-peptide A, QD-peptide B, which binds peptide A (ZhangKodadek2000NatBiotech)
  • AMPAR-unnatural amino acid azide, QD-propargyl (ChaterjeeSchultz2013PNAS)

Protein Interaction

  • FRET
  • Two Hybrid Screening

Cell Morphology

Controlling Neural Activity

  • Optogenetics
  • Protein Uncaging

Gene Related Assays

  • TET/DOX transcription regulation
  • CRE/LOX

Pages in category "Neuroscience Methods"

The following 5 pages are in this category, out of 5 total.

B

C

M

P

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