Molecular Methods

Malinow

Molecular Methods

Quantum Dots

Choquet

AMPAR

Zac Email

Hi Guys, Here are the different labeling techniques that might be applicable with recombinant expression of AMPARs. Roughly ranked from most to least likely to succeed, separated by large vs small AMPAR N-terminal additions. The references in parentheses are for background on the technique.

Large AMPAR N-terminal addition

• Halotag (Promega): AMPAR-enzyme, QD-Halotag substrate
• AMPAR-streptavidin, QD-biotin

Small AMPAR N-terminal addition

• AMPAR-FLAG, QD-anti-FLAG
• AMPAR-biotin ligase recognition peptide, QD-biotin + biotin ligase (LuTing2013PLOSONE)
• AMPAR-peptide A, QD-peptide B, which binds peptide A (ZhangKodadek2000NatBiotech)
• AMPAR-unnatural amino acid azide, QD-propargyl (ChaterjeeSchultz2013PNAS)

Fluorescence Imaging

Molecular Interaction or Proximity

• FRET or BRET
• BiFC

Microscopy

• PALM STORM
• Fluorescence-lifetime imaging microscopy or FLIM
• Two-photon Microscopy

See Also

• Fluorescence applications in molecular neurobiology