Molecular Methods: Difference between revisions
Jump to navigation
Jump to search
Bradley Monk (talk | contribs) No edit summary |
Bradley Monk (talk | contribs) No edit summary |
||
| (3 intermediate revisions by the same user not shown) | |||
| Line 1: | Line 1: | ||
{{PageHead|[[Malinow]]|[[Molecular Methods]]|[[Quantum Dots]]|[[Choquet]]|[[AMPAR]]}} | |||
==Zac Email== | |||
Hi Guys, | |||
Here are the different labeling techniques that might be applicable with recombinant expression of AMPARs. Roughly ranked from most to least likely to succeed, separated by large vs small [[AMPAR]] N-terminal additions. The references in parentheses are for background on the technique. | |||
:'''Large [[AMPAR]] N-terminal addition''' | |||
:* Halotag (Promega): [[AMPAR]]-enzyme, QD-Halotag substrate | |||
:* [[AMPAR]]-streptavidin, QD-biotin | |||
:'''Small [[AMPAR]] N-terminal addition''' | |||
:* [[AMPAR]]-FLAG, QD-anti-FLAG | |||
:* [[AMPAR]]-biotin ligase recognition peptide, QD-biotin + biotin ligase (LuTing2013PLOSONE) | |||
:* [[AMPAR]]-peptide A, QD-peptide B, which binds peptide A (ZhangKodadek2000NatBiotech) | |||
:* [[AMPAR]]-unnatural amino acid azide, QD-propargyl (ChaterjeeSchultz2013PNAS) | |||
==Fluorescence Imaging== | ==Fluorescence Imaging== | ||
| Line 10: | Line 27: | ||
:* [http://en.wikipedia.org/wiki/Super_resolution_microscopy#Photoactivated_localization_microscopy_.28PALM.29_and_FPALM PALM STORM] | :* [http://en.wikipedia.org/wiki/Super_resolution_microscopy#Photoactivated_localization_microscopy_.28PALM.29_and_FPALM PALM STORM] | ||
:* [http://en.wikipedia.org/wiki/Fluorescence-lifetime_imaging_microscopy Fluorescence-lifetime imaging microscopy or FLIM] | :* [http://en.wikipedia.org/wiki/Fluorescence-lifetime_imaging_microscopy Fluorescence-lifetime imaging microscopy or FLIM] | ||
:* [http://en.wikipedia.org/wiki/Two-photon_excitation_microscopy Two-photon Microscopy] | |||
| Line 28: | Line 46: | ||
[[Category:Neuroscience Methods]] | [[Category:Neuroscience Methods]] [[Category:ReDiClus]][[Category:Neurobiology]] | ||
Latest revision as of 22:20, 9 August 2014
| Malinow | Molecular Methods | Quantum Dots | Choquet | AMPAR |
Zac Email
Hi Guys, Here are the different labeling techniques that might be applicable with recombinant expression of AMPARs. Roughly ranked from most to least likely to succeed, separated by large vs small AMPAR N-terminal additions. The references in parentheses are for background on the technique.
- Large AMPAR N-terminal addition
- Small AMPAR N-terminal addition
Fluorescence Imaging